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CURRICULUM VITAE ET STUDIORUM

Alessandra Gambacurta

Dati Anagrafici

Luogo e data di nascita: Roma, 22 giugno 1960

Recapito Ufficio: Tel. +39-06-72596488

e-mail: gambacur@uniroma2.it

Indirizzo Ufficio: Dipartimento di Medicina Sperimentale, Università "Tor Vergata"

Via Montpellier, 1 – 00133, Roma

Studi e carriera universitaria

1985 - Laureata in Scienze Biologiche con lode, Università degli Studi di Roma La Sapienza

1986 - Abilitata all’esercizio della professione di Biologo

1988-1989 Borsista presso il Max Planck Institut fur Molekulare Genetik, Berlino

1992 - Dottore di ricerca in Biochimica, Università degli Studi di Roma La Sapienza

1994-oggi: Ricercatore di Biologia Molecolare (settore scientifico disciplinare BIO 11) presso il Dipartimento di Medicina Sperimentale dell’Università di Roma Tor Vergata

2001 - Abilitazione Scientifica Nazionale come professore associato, SSD BIO/11, settore concorsuale 05/E2

2020 - Abilitazione Scientifica Nazionale come professore associato, SSD BIO/11, settore concorsuale 05/E2 (scadenza 13/11/2029)

2022 - Professore associato SSD BIOS-08/A.

Pubblicazioni scientifiche

Alessandra Gambacurta è autrice di circa 43 pubblicazioni su riviste internazionali.

Ente

H-index

dal 2011

Citazioni

dal 2011

Articoli

dal 2011

Google Scholar

672

515

Scopus

450

310

WOS

470

257

Campi di attività scientifica - linee di ricerca odierne

Studio dei meccanismi molecolari ed epigenetici alla base della deprogrammazione cellulare di cellule nucleate del sangue;
Studio dei meccanismi di differenziamento in cellule staminali derivate dal sangue (BDSC);
Applicazione delle cellule staminali derivate da sangue in medicina veterinaria (cavalli, cani, gatti) su patologie non indotte, con particolare riguardo a tendini, muscoli e ossa;
Riprogrammazione di cellule tumorali in cellule normali con particolare attenzione ai meccanismi molecolari ed epigenetici coinvolti nel transdifferenziamento;
Ruolo del metabolismo energetico nella riprogrammazione delle cellule tumorali differenziate, individuazione della cascata molecolare (epigenetica e proteica) responsabile del cambiamento metabolico;
Studio del “rimodellamento cromatinico” con riguardo alle relazioni tra modifiche epigenetiche, regolazione dell’espressione genica e cambiamenti metabolici\morfologici delle cellule tumorali e staminali;
Identificazione di marcatori epigenetici per una diagnosi tumorale precoce;
Sviluppo di un lab-on-chip (microfluidic device) per la rilevazione di cellule tumorali in fluidi biologici.

Fondi di ricerca

anno

ente

titolo

finanziamento

note

2006-2008

Ministero della

Ricerca Scientifica

Progetto PRIN

(protocollo 2005052299_001)

Studiodell’interazione lipossigenasi-lipidi: analisi spettroscopica dei cambiamenti conformazionali proteici, delle proprietà dinamiche dei lipidi e delle proprietà elastiche del sistema

Partecipante

2008-2010

Ministero della

Ricerca Scientifica

Progetto PRIN

(2007KAWXCL_002)

Studi strutturali e di stabilità di mutanti del citocromo c da utilizzare in area biosensoristica

25000 Euro

Partecipante

2006-2014

Conto terzi

Messa a punto del protocollo di ottenimento di cellule staminale da sangue periferico

-Euro18.000\anno in consumabili

-Euro 600.000 apparecchiature: FACSAria, FACSCalibur

2014- oggi

Centro NAST

(fondo libere donazioni)

Progetto cellule staminali

circa 60.000 Euro

2017-2019

ASI

progetto “Serism” – BioMission VITA

(2016-5-U.0)

200.000 Euro

CoPI

2018-oggi

FESR – Fondo Europeo di Sviluppo Regionale

progetto Lactogene

(POR 2014-2020)

90.000 Euro

Brevetti internazionali

Kit for collecting blood, preferably peripheral blood, for the production of stem cells

Patent number: 8500712

Inventors: Marco Polettini, Alessandra Gambacurta

Abstract: A kit for collecting blood, preferably peripheral blood, for the production of pluripotent stem cells comprises at least a first container, able to contain the blood taken, which contains an anticoagulant and the substance MCSF (Macrophage Colony Stimulating Factor).

Type: Grant

Filed: March 17, 2009

Date of Patent: August 6, 2013

2) Pharmaceutical Composition Containing Expanded Adult Stem Cells and Methods of Using Same for Treatment

Publication number: 20120308535

Inventors: Alessandra GAMBACURTA, Marco POLETTINI

Abstract: A method for the expansion of adult stem cells from blood, particularly but not only peripheral blood, involves removing adult stem cells from blood of a mammal, immediately expanding the stem cells via in-vitro treatment with MCSF (Macrophage Colony Stimulating Factor) at a concentration of about 8-15 nM, and purifying the expanded stem cells. Compositions and methods of using the expanded adult stem cells are also described.

Type: Application

Filed: August 16, 2012

Publication date: December 6, 2012

3) Method for expanding adult stem cells from blood and compositions and methods for using the same

Patent number: 8263400

Inventors: Alessandra Gambacurta, Marco Polettini

Type: Grant

Filed: March 20, 2009

Date of Patent: September 11, 2012

Innovazione - Startup

2006 - socia del progetto-laboratorio interfacoltà “NAST” di Tor Vergata
2018 – Co-fondatrice e Responsabile scientifico della start up: Orionetix s.r.l.

Orionetix (https://www.orionetix.com/) è una StartUp innovativa fondata nel 2018 da un team di giovani ricercatori che opera nell’ambito della biologia molecolare e dell’ingegneria elettronica. Si occupa di ricerca, sviluppo, produzione e commercializzazione di prodotti e servizi innovativi ad alto valore tecnologico nell’ambito delle biotecnologie, della biosensoristica e della diagnostica molecolare e dell’identificazione di metodi, prodotti e materiali innovativi relativi al settore ambientale, del lavoro, della salute, del benessere e della sicurezza. Orionetix sviluppa algoritmi di intelligenza artificiale e prototipi che permettono all’utente di svolgere operazioni complesse di biologia molecolare e/o cellulare in maniera semplice e automatizzata, senza necessità di conoscenze professionali, specialistiche o di attrezzatura di laboratorio.

Collaborazioni scientifiche

Collaborazioni in ambito internazionale e nazionale:

Professor G. N. La Mar, Dept. of Chemistry, University of California, Davis, USA;
Professor W.E. Royer, University of Massachussetts, Usa;
Professor J. Friedman Albert Einstein College of Medicine, New York, USA;
Professor Van De Walle Gerlinde Veterinary University of Ghent, Belgio;
Professor Deprez Piet Veterinary University of Ghent Belgio;
Professor Mauro Maccarrone Campus Biomedico, Roma;
Professoressa Anna Maria Sardanelli, Università di Bari;
Dr.ssa Maria Lucibello CNR, Roma.

Incarichi universitari e ministeriali

2020 – presente: Componente Giunta della Facoltà di Medicina e Chirurgia Facoltà

2014 – presente: iscritta all’albo dei revisori Reprise del Ministero dell’Università e della Ricerca

Accordi InternazionaliUniversità Tor Vergata

Anno

Responsabile

Facoltà

Nazione

Istituzione

Data scadenza

2010

Gambacurta Alessandra

Medicina e Chirurgia

Belgium

Universiteit Ghent

2014-08-12

Revisore Riviste Scientifiche

Attività di revisore per le seguenti riviste scientifiche:

Life Sciences (Elsevier)
Biochemical Journal (Portland Press)
Cells (MDPI)
Equine Veterinary Journal (Wiley)
Equine Veterinary Journal and Education (Wiley)

Partecipazione a scuole di dottorato

2009-2011 Membro del Collegio dei Docenti del Dottorato di Ricerca “Advanced technology in rehabilitation medicine and sport”. ” Università di Roma “Tor Vergata”.

2017-ad oggi Membro del Collegio dei Docenti del Dottorato di Ricerca in “Biologia Molecolare e Biochimica” Università di Roma “Tor Vergata”.

Attività Didattica I: corsi di Laurea TriennaliUniversità Tor Vergata

anni

Insegnamento

c.f.u.

#studenti

CL triennale

2001- oggi

Biologia molecolare

≈ 65

Tecniche di Laboratorio Biomedico

2001-oggi

Biologia molecolare

≈ 270

Ingegneria Medica

Attività Didattica II: corso di Laurea Ciclo Unico, Medicina e Chirurgia Università Tor Vergata

anni

Insegnamento

c.f.u.

# studenti

Note

2014-2016

Molecular Biology

≈ 30

Corso di Laurea in lingua inglese

2016-2018

Biologia Molecolare

≈ 200

Corso di laurea

Attività Didattica III: corsi di Specializzazione Università Tor Vergata

anni

Insegnamento

c.f.u.

#studenti

Scuola Specializzazione

2011- 2013

Tecniche di DNA Ricombinante

≈ 20

Microbiologia e Virologia Clinica

Attività Didattica IV: Esami di profitto di Biochimica/

Biologia Molecolare/Chimica Biologica svolti all’Università di Tor Vergata

(PS. la tabella è limitata al 2014, in quanto primo anno di registrazione digitale dei verbali)

A.A.

# verbali totali

2014-2015

113

2015-2016

285

2016-2017

228

2017-2018

341

2018-2019

310

2019-2020

355

2020-2021

195

Totale 1827

Attività Didattica V: Relatore tesi Corsi di laurea e Dottorato di Ricerca svolte all’Università di Tor Vergata

(PS. la tabella è limitata al 2007, in quanto primo anno di registrazione digitale)

A.A.

Relatore tesi

2007-2008

2009-2010

2010-2011

2012-2013

2013-2014

2014-2015

2016-2017

2017-2018

2020-2021

Totale 21

Segue la lista completa delle pubblicazioni di Alessandra Gambacurta

Si dichiara, come richiesto dal bando (Decreto rettorale n. 302 del 8/02/2022 - Rif. n. 1863,) che: tutto quanto è stato dichiarato in questo CV corrisponde a verità ai sensi degli articoli 46 e 47 del D.P.R. 445 del 2000.

Roma, 18 Giugno 2024

Elenco delle pubblicazioni di Alessandra Gambacurta

Total papers

First author

Last author

Corresponding author

“Partial Loss of Function ABCA12 Mutations Generate Reduced Deposition of Glucosyl-Ceramide, Leading to Patchy Ichthyosis and Erythrodermia Resembling Erythrokeratodermia Variabilis et Progressiva (EKVP)”

Terrinoni A., Sala G., Bruno E., Pitolli C., Minieri M., Pieri M., Gambacurta A., Campione E., Belardi R., Bernardini S. (2023)

Int J Mol Sci. 24(18), 13962. IF 5.6

“In Vivo Identification of H3K9me2/H3K79me3 as an Epigenetic Barrier to Carcinogenesis”.

Piro M.C., Gasperi V., De Stefano A., Anemona L., Cenciarelli C.R, Mauriello A., Montanaro M., Catani M.V., Terrinoni A., and Gambacurta A. (2023)

Int J Mol Sci.24(15), 12158. IF 5.6

“DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines”.

D'Amico S, Krasnowska EK, Manni I, Toietta G, Baldari S, Piaggio G, Ranalli M, Gambacurta A, Vernieri C, Di Giacinto F, Bernassola F, de Braud F, Lucibello M. (2020).

Cells.19;9(5):1260. IF 6,6, Citazioni: 5

“Opening the Gate to the Serism Project: From Earth to Space and Back”.

M. Maccarrone*, M. Fava, N. Battista, S. Piccirillo, G. Valentini, G. Mascetti, A. Gambacurta * & M. Bari*, (2020).

Aerotecnica Missili & Spazio Volume 99, Issue 2, *co-corrispondentiCitazioni: 1

“Microcalcifications Drive Breast Cancer Occurrence and Development by Macrophage-Mediated Epithelial to Mesenchymal Transition”.

Scimeca M, Bonfiglio R, Menichini E, Albonici L, Urbano N, De Caro MT, Mauriello A, Schillaci O, Gambacurta A, Bonanno E. (2019).

Int J Mol Sci.11;20(22):5633. IF 4.556.Citazioni: 20

“Cell death in cancer in the era of precision medicine”

Raschellà, G., Melino, G. & Gambacurta, A. (2019).

Genes Immun.20(7):529-538. IF 2.96 Citazioni: 8

“Human osteogenic differentiation in Space: proteomic and epigenetic clues to better understand osteoporosis”

Gambacurta, A.,Merlini, G., Ruggiero, C. Diedenhofen G., Battista N., Bari M., Balsamo M., Piccirillo S., Valentini G., Mascetti G. & Maccarrone M. (2019).

Sci Rep 9, 8343. IF 4.16. Citazioni: 19

“Human endogenous retrovirus K in the crosstalk between cancer cells microenvironment and plasticity: a new perspective for combination therapy”

E Balestrieri, A Argaw-Denboba, A Gambacurta,C. Cipriani, R. Bei, A. Serafino, P. Sinibaldi-Vallebona and C. Matteucci (2018).

Front. Microbiol. 2;9:1448. IF 5,26. Citazioni: 15

“Challenging tumor resistance with less toxic, more effective drug combinations: an example from neuroblastoma”.

A Gambacurta, G Raschellà (2018)

Cell Death Dis. 9, 686. IF 8,46. Citazioni: 1

“Myoblasts rely on TAp63 to control basal mitochondria respiration”

Ciuffoli V, Lena AM, Gambacurta A, Melino G, Candi E. (2018).

Aging(Albany NY). 28;10(11):3558-3573. IF 5,68. Citazioni: 1

“TAp73 regulates ATP7A: possible implications for ageing-related diseases”.

Lopriore P, Capitanio N, Panatta E, Di Daniele N, Gambacurta A, Melino G, Amelio I. (2018)

Aging(Albany NY). 8;10(12):3745-3760. IF 5,68. Citazioni: 2

“Rapid Rapamycin-Only Induced Osteogenic Differentiation of Blood-Derived Stem Cells and Their Adhesion to Natural and Artificial Scaffolds”.

Carpentieri A., Cozzoli E., Acri F., Ranalli M., Diedenhofen G., Scimeca M., Bonanno E.Gambacurta A.(2017).

Stem Cells Int. 2017:2976541. IF 5,44. Citazioni: 4

“HERV-K activation is strictly required to sustain CD133+ melanoma cells with stemness features”.

Argaw-Denboba, A., Balestrieri, E., Serafino, A., Cipriani, C., Bucci, I., Sorrentino, R.,SciamannaI, Gambacurta, A, Sinibaldi-VallebonaP,Matteucci, C. (2017)

Exp Clin Cancer Res. 26;36(1):20. IF 9,95. Citazioni: 33

“Back on Track: New Perspectives on Cancer Cell Reprogramming”.

Carpentieri Arianna, Didenhofen Giacomo, Gambacurta Alessandra. (2016)

Single Cell Biol 5:3 IF 2,57. Citazioni: 1

“Differentiation of human neuroblastoma cells towards the osteogenic lineage by mTOR inhibitor”.

Carpentieri A, Cozzoli E, Scimeca M, Bonanno E, Sardanelli AM, Gambacurta A. (2015)

Cell Death Dis. 6(11):e1974. IF 8,46. Citazioni: 17

“Blood-Derived Stem Cells (BDSCs) Plasticity: In Vitro Hepatic Differentiation”.

Alaimo G, Cozzoli E, Marfe G, Esposito L, Ranalli M, Hmada D, Giordano A and Gambacurta A.(2013)

J Cell Physiol. 228(6):1249-54. IF 6,38. Citazioni: 8

“IL-25 prevents and cures fulminant hepatitis in mice through a myeloid-derived suppressor cell-dependent mechanism”.

Sarra, M., Cupi, M. L., Bernardini, R., Ronchetti, G., Monteleone, I., Ranalli, M., Franzè, E., Rizzo, A., Colantoni, A., Caprioli, F., Maggioni, M., Gambacurta, A., Mattei, M., Macdonald,T., Pallone, F. and Monteleone, G. (2013)

Hepatology. 58(4):1436-50. IF 17,4. Citazioni: 33

“A new clinical approach: use of blood-derived stem cells (BDSCs) for superficial digital flexor tendon injuries in horses”.

Marfe G, Rotta G, De Martino L, Tafani M, Fiorito F, Di Stefano C, Polettini M, Ranalli M, Russo MA, Gambacurta A. (2012)

Life Sci. 6;90(21-22):825-30. IF 5,0. Citazioni: 29

“Blood derived stem cells: An ameliorative therapy in veterinary ophthalmology”.

Marfe, G., Massaro-Giordano, M., Ranalli, M., Cozzoli, E., Di Stefano, C., Malafoglia, V., Polettini, M. and Gambacurta, A. (2012)

J Cell Physiol. 227(3):1250-6. IF 6,38. Citazioni: 22

“A Multicancer-Like Syndrome in A Dog Characterized By P53 and Cell Cycle-Checkpoint Kinase 2 (Chk2) Mutations and Sirtuin Gene (Sirt1) Down-Regulation”.

G Marfe, L De Martino, M Tafani, M Irno-Consalvo, Mp Pasolini, L Navas, A. Gambacurta. O. Paciello. (2012)

Res Vet Sci. 93(1):240-5. IF 2,53. Citazioni: 8

“The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise”.

G Marfe, V Manzi, M Tafani, B Pucci, A Gambacurta, M Russo. (2012)

Open Journal of Molecular and Integrative Physiology, 2, 65-74. IF 0,4. Citazioni: 5

“ATP Regulation of the Ligand-Binding Properties in Temperate and Cold-Adapted Haemoglobins. X-Ray Structure And Ligand-Binding Kinetics In The Sub-Antarctic Fish Eleginops”

Coppola D, Abbruzzetti S, Nicoletti F, Merlino A, Gambacurta A, Giordano D, Howes BD, De Sanctis G, Vitagliano L, Bruno S, di Prisco G, Mazzarella L, Smulevich G, Coletta M, Viappiani C, Vergara A, Verde C. (2012)

Mol Biosyst. 30;8(12):3295-304. IF 3,49. Citazioni: 11

“Purification and expansion of stem cells from equine peripheral blood, with clinical applications”

J. Spaas, A. Gambacurta, M. Polettini, S. Broeckx, F. Van Hoeck, C. De Schauwer, G. Van de Walle (2011).

VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT, vol. 80; p. 129-135. IF 0,369. Citazioni: 15

“TCTP is a critical survival factor that protects cancer cells from oxidative stress-induced cell-death”.

Lucibello M, Gambacurta A., Zonfrillo M, Pierimarchi P, Serafino A, Rasi G, Rubartelli A, Garaci E. (2011)

Exp Cell Res. 15;317(17):2479-89. IF 3,55. Citazioni: 36

“Sphingosine Kinase 1 Overexpression Is Regulated By Signaling Through Pi3k, Akt2, And mTOR In Imatinib-Resistant Chronic Myeloid Leukemia Cells”.

Marfe G, Di Stefano C, Gambacurta A, Ottone T, Martini V, Abruzzese E, Mologni L, Sinibaldi-Salimei P, de Fabritis P, Gambacorti-Passerini C, Amadori S, Birge RB. (2011)

Exp Hematol. 39(6):653-665.e6. IF 3,08. Citazioni: 37

“Ultrasound Prediction Of The Partus Datum In Dogs”

P De Wispelaere, A Van Soom, T Rijsselaere, J Saunders, G Storms, A Gambacurta, F. Haesebrouck .(2011)

Tijdschrift Voor Diergeneeskunde 136 (6), 418-420. IF 0,188. Citazioni: -

“A new T677C mutation of the aspartoacylase gene encodes for a protein with no enzymatic activity”

Di Pietro V, Gambacurta A, Amorini AM, Finocchiaro A, D'Urso S, Ceccarelli L, Tavazzi B, Giardina B, Lazzarino G(2008).

Clin Biochem. 41(7-8):611-5. IF 3,28. Citazioni: 5

“rhEPO (Recombinant Human Eosinophil Peroxidase): Expression in Pichia Pastoris and Biochemical Characterization”

C Ciaccio, A Gambacurta, G. de Sanctis, D Spagnolo, C Sakarikou, M. Coletta. (2006).

Biochem J. 15;395(2):295-301. IF 4,4. Citazioni: 7

“Bovine Tryptases: cDNA cloning, tissue specific expression and characterization of the lung isoform”

A Gambacurta, L Fiorucci, P Basili, F Erba, A Amoresano, F Ascoli. (2003).

Eur J Biochem. 270(3):507-17. Title changed to 'FEBS Journal' since2005IF 4,67. Citazioni: 3

“Structure of the Fe-Heme In The Homodimeric Hemoglobin From Scapharca Inaequivalvis And In The T72i Mutant: An X-Ray absorption spectroscopic study at low temperature”.

S Della Longa, A Gambacurta, A Bertollini, M Girasole, A Castellano. (2001)

European Biophys J. 2001;29(8):559-68. IF 1,733. Citazioni: 2

“Scapharca Inaequivalvis A And B Miniglobin Genes: Promoter Activity Of The 5′ Flanking Regions And In Vivo Transcription”.

A Gambacurta, P Basili, F Ascoli. (2000)

Gene. 2000 Sep 5;255(1):75-81. IF 3,47. Citazioni: 3

“Functional Modulation of The Thr72→Ile Mutant From Scapharca Inaequivalvis homodimeric Hemoglobin”. M Coletta, A Gambacurta, Me Clementi, F Erba, F Polizio, M Falconi , F. Ascoli (1999)

JBIC Journal of Biological Inorganic Chemistry 4 (6), 678-683IF 3,2. Citazioni: 2

“Fe-Heme Structure And Dynamics In Thr72→ Ile Mutant Scapharca Inaequivalvis Hemoglobin By X-Ray Absorption Spectroscopy”.

S Della Longa, A Gambacurta, I Ascone, A Bertollini, M Girasole. (1999)

Journal Of Synchrotron Radiation 6 (3), 392-393IF 0,67. Citazioni: 2

“Mutational destabilization of the critical interface water cluster in scapharca dimeric hemoglobin: structural basis for altered allosteric activity”.

A Pardanani, A Gambacurta, F Ascoli, W Royer Jr. (1998)

Journal of Molecular Biology 284 (3), 729-739IF 5,1. Citazioni: 29

“Structural And Dynamic Properties Of The Homodimeric Hemoglobin From Scapharca Inaequivalvis Thr-72→ Ile Mutant: Molecular Dynamics Simulation, Low Temperature Visible”.

M Falconi, A Desideri, A Cupane, M Leone, G Ciccotti, Es Peterson, A.Gambacurta, F. Ascoli. (1998)

Biophysical Journal 75 (5), 2489-2503IF 3,4. Citazioni: 6

“The Exon/Intron Organization Of The Globin Gene Of Scapharca Inaequivalvis Homodimeric Hemoglobin: Unusual Intron Homology With Other Bivalve Mollusc Globin Genes”.

Mc Piro, A Gambacurta, P Basili, F Ascoli. (1998)

Gene 221 (1), 45-49IF 3,47. Citazioni: 2

“Scapharca Inaequivalvis Tetrameric Hemoglobin A and B Genes: Evidence for a Minigene”.

A Gambacurta, Mc Piro, F Ascoli. (1998)

Journal Of Molecular Evolution 47 (2), 167-171IF 2,3. Citazioni: 4

“Proton-NMR Investigation Of The Heme Cavity In The Cyanomet Derivative Of The Cooperative Homodimeric Hemoglobin From Scapharca Inaequivalvis”.

Y Wu, M Basti, A Gambacurta, E Chiancone, F Ascoli, G. La Mar. (1996)

Biochimica et Biophysica Acta (Bba)-Protein Structure And Molecular Enzymology 8, 94IF 3,0. Citazioni: 11

“Scapharca Inaequivalvis Tetrameric Hemoglobin A and B Chains: cDNA Sequencing and Genomic Organization”.

Mc Piro, A Gambacurta, F Ascoli. (1996)

Journal Of Molecular Evolution 43 (6), 594-601IF 2,3. Citazioni: 10

“cDNA cloning and primary structure of tryptase from bovine mast cells, and evidence for the expression of bovine pancreatic trypsin inhibitor mRNA in the same cells”.

M Pallaoro, A Gambacurta, L Fiorucci, G Mignogna, D Barra, F Ascoli. (1996)

European Journal Of Biochemistry 237 (1), 100-105IF 4,67. Citazioni: 19

“A single mutation (Thr72 → IIe) at the subunit interface is crucial for the functional properties of the homodimeric co-operative haemoglobin from Scapharca inaequivalvis”.

Gambacurta A., Piro M.C., Coletta M., Clementi M.E., Polizio F., Desideri A., Santucci R., Ascoli F. (1995).

J. Mol. Biol, 248, 910-917. IF 5,1. Citazioni: 10

“Differential in vitro translation of the precursors of bovine pancreatic trypsin inhibitor and its isoinhibitor II is controlled by the 5′-end region of their mRNAs”.

Gambacurta, A., Piro, M.C., Ascoli, F.(1993)

BBA - Gene Structure and Expression, 1174 (3), pp. 267-273. IF 4,72. Citazioni: 2

“Cooperative homodimeric hemoglobin from Scapharca inaequivalvis. cDNA cloning and expression of the fully functional protein in E. Coli “.

Gambacurta, A., Piro, M.C., Ascoli, F. (1993)

FEBS Letters, 330 (1), pp. 90-94. IF 4,12. Citazioni: 23

“Modulation of Adriamycin Uptake By Lonidamine In Ehrlich Ascites Tumor Cells”.

A Floridi, A Gambacurta, A Bagnato, C Bianchi, Mg Paggi, B Silvestrini. (1988)

Experimental and Molecular Pathology 49 (3), 421-431IF 3,3. Citazioni: 23

“Lonidamine-Induced Outer Membrane Permeability and Susceptibility of Mitochondria to inhibition by Adriamycin”.

A Floridi, C Bianchi, A Bagnato, A Gambacurta, Mg Paggi, B Silvestrini. (1987)

Anticancer Research 7 (6), 1149-1152IF 2,4. Citazioni:

CURRICULUM VITAE

PRESENT POSITION: Researcher (since 1994) in Molecular Biology at the Department of Experimental Medicine and Surgery of the University of Rome “Tor Vergata”, Rome. Italy.

EDUCATION: Degree in Biological Sciences, Qualified as a Biologist,

PhD in Biochemistry.

PREVIOUS WORK:

1987-1988 Fellowship at the Max Planck Institut fur Molekulare Genetik, Berlin

1989-1992 PhD in Biochemistry, University of Rome "Tor Vergata".

1994-today Researcher BIO/11 Faculty of Medicine and Surgery, University of Rome "Tor Vergata".

2001 Obtaining qualification of Associate Professor BIO/11

2022 - Associate Professor SSD BIOS-08/A

EDITORIAL ACTIVITY:

2012 - today Member of the Faculty Board of the PhD in Molecular Biology and Biochemistry

2014- today Referee for MIUR for scientific projects

2005 - today Referee for several international journals

Life Sciences

Biochemical journal

Equine Veterinary Journal

Equine Veterinary Journal and Education

RESEARCH INTERESTS:

- Study of the molecular and epigenetic mechanisms underlying cell deprogramming

- Study of the mechanisms of stem cells differentiation

- Application of blood stem cells in veterinary medicine (horses, dogs, cats), with particular regard to tendons, muscles and bones

- Reprogramming tumor cells in normal cells with particular attention to the molecular and epigenetic mechanisms responsible for the return of tumor cells to normal cells.

-Role of energy metabolism in reprogramming in differentiated tumor cells, identification of the molecular cascade responsible for metabolic change

FINANCED RESEARCH PROJECTS AND OTHER RESEARCH FUNDING:

• 2006-2008 Prin Project, protocol 2005052299_001, Study of lipoxygenase-lipid interaction: spectroscopic analysis of protein conformational changes, of the dynamic properties of lipids and of the elastic properties of the system (participant)

• 2008-2010 Prin Project, protocol 2007KAWXCL_002, Structural and stability studies of cytochrome c mutants to be used in the biosensoristic area (participant).

• Third-party account 2006-2014 "Development of the protocol to obtain stem cells from peripheral blood"

• 2014 - to date: Responsible "Stem Cell Project" Centro NAST, University of Rome "Tor Vergata".

• 2017: ASI - Project "Serism" - BioMissione VITA (Participant, creator and manager of the experimental model of osteogenesis brought to the ISS)

INTERNATIONAL AND EUROPEAN PATENTS:

Kit for collecting blood, preferably peripheral blood, for the production of stem cells

Patent number: 8500712

Inventors: Marco Polettini, Alessandra Gambacurta

Type: Grant

Filed: March 17, 2009

Date of Patent: August 6, 2013

2) Pharmaceutical Composition Containing Expanded Adult Stem Cells and Methods of Using Same for Treatment

Publication number: 20120308535

Inventors: Alessandra GAMBACURTA, Marco POLETTINI

Type: Application

Filed: August 16, 2012

Publication date: December 6, 2012

3) Method for expanding adult stem cells from blood and compositions and methods for using the same

Patent number: 8263400

Inventors: Alessandra Gambacurta, Marco Polettini

Type: Grant

Filed: March 20, 2009

Date of Patent: September 11, 2012

Elenco delle pubblicazioni di Alessandra Gambacurta

Total papers

First author

Last author

Corresponding author

Terrinoni A., Sala G., Bruno E., Pitolli C., Minieri M., Pieri M., Gambacurta A., Campione E., Belardi R., Bernardini S. (2023)

Int J Mol Sci. 24(18), 13962. IF 5.6

Piro M.C., Gasperi V., De Stefano A., Anemona L., Cenciarelli C.R, Mauriello A., Montanaro M., Catani M.V., Terrinoni A., and Gambacurta A. (2023)

Int J Mol Sci.24(15), 12158. IF 5.6

D'Amico S, Krasnowska EK, Manni I, Toietta G, Baldari S, Piaggio G, Ranalli M, Gambacurta A, Vernieri C, Di Giacinto F, Bernassola F, de Braud F, Lucibello M. (2020).

Cells.19;9(5):1260. IF 6,6, Citazioni: 5

M. Maccarrone*, M. Fava, N. Battista, S. Piccirillo, G. Valentini, G. Mascetti, A. Gambacurta * & M. Bari*, (2020).

Aerotecnica Missili & Spazio Volume 99, Issue 2, *co-corrispondentiCitazioni: 1

Scimeca M, Bonfiglio R, Menichini E, Albonici L, Urbano N, De Caro MT, Mauriello A, Schillaci O, Gambacurta A, Bonanno E. (2019).

Int J Mol Sci.11;20(22):5633. IF 4.556.Citazioni: 20

Raschellà, G., Melino, G. & Gambacurta, A. (2019).

Genes Immun.20(7):529-538. IF 2.96 Citazioni: 8

Gambacurta, A.,Merlini, G., Ruggiero, C. Diedenhofen G., Battista N., Bari M., Balsamo M., Piccirillo S., Valentini G., Mascetti G. & Maccarrone M. (2019).

Sci Rep 9, 8343. IF 4.16. Citazioni: 19

E Balestrieri, A Argaw-Denboba, A Gambacurta,C. Cipriani, R. Bei, A. Serafino, P. Sinibaldi-Vallebona and C. Matteucci (2018).

Front. Microbiol. 2;9:1448. IF 5,26. Citazioni: 15

A Gambacurta, G Raschellà (2018)

Cell Death Dis. 9, 686. IF 8,46. Citazioni: 1

Ciuffoli V, Lena AM, Gambacurta A, Melino G, Candi E. (2018).

Aging(Albany NY). 28;10(11):3558-3573. IF 5,68. Citazioni: 1

Lopriore P, Capitanio N, Panatta E, Di Daniele N, Gambacurta A, Melino G, Amelio I. (2018)

Aging(Albany NY). 8;10(12):3745-3760. IF 5,68. Citazioni: 2

Carpentieri A., Cozzoli E., Acri F., Ranalli M., Diedenhofen G., Scimeca M., Bonanno E.Gambacurta A.(2017).

Stem Cells Int. 2017:2976541. IF 5,44. Citazioni: 4

Argaw-Denboba, A., Balestrieri, E., Serafino, A., Cipriani, C., Bucci, I., Sorrentino, R.,SciamannaI, Gambacurta, A, Sinibaldi-VallebonaP,Matteucci, C. (2017)

Exp Clin Cancer Res. 26;36(1):20. IF 9,95. Citazioni: 33

Carpentieri Arianna, Didenhofen Giacomo, Gambacurta Alessandra. (2016)

Single Cell Biol 5:3 IF 2,57. Citazioni: 1

Carpentieri A, Cozzoli E, Scimeca M, Bonanno E, Sardanelli AM, Gambacurta A. (2015)

Cell Death Dis. 6(11):e1974. IF 8,46. Citazioni: 17

Alaimo G, Cozzoli E, Marfe G, Esposito L, Ranalli M, Hmada D, Giordano A and Gambacurta A.(2013)

J Cell Physiol. 228(6):1249-54. IF 6,38. Citazioni: 8

Hepatology. 58(4):1436-50. IF 17,4. Citazioni: 33

Marfe G, Rotta G, De Martino L, Tafani M, Fiorito F, Di Stefano C, Polettini M, Ranalli M, Russo MA, Gambacurta A. (2012)

Life Sci. 6;90(21-22):825-30. IF 5,0. Citazioni: 29

Marfe, G., Massaro-Giordano, M., Ranalli, M., Cozzoli, E., Di Stefano, C., Malafoglia, V., Polettini, M. and Gambacurta, A. (2012)

J Cell Physiol. 227(3):1250-6. IF 6,38. Citazioni: 22

G Marfe, L De Martino, M Tafani, M Irno-Consalvo, Mp Pasolini, L Navas, A. Gambacurta. O. Paciello. (2012)

Res Vet Sci. 93(1):240-5. IF 2,53. Citazioni: 8

G Marfe, V Manzi, M Tafani, B Pucci, A Gambacurta, M Russo. (2012)

Open Journal of Molecular and Integrative Physiology, 2, 65-74. IF 0,4. Citazioni: 5

Mol Biosyst. 30;8(12):3295-304. IF 3,49. Citazioni: 11

J. Spaas, A. Gambacurta, M. Polettini, S. Broeckx, F. Van Hoeck, C. De Schauwer, G. Van de Walle (2011).

VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT, vol. 80; p. 129-135. IF 0,369. Citazioni: 15

Lucibello M, Gambacurta A., Zonfrillo M, Pierimarchi P, Serafino A, Rasi G, Rubartelli A, Garaci E. (2011)

Exp Cell Res. 15;317(17):2479-89. IF 3,55. Citazioni: 36

Marfe G, Di Stefano C, Gambacurta A, Ottone T, Martini V, Abruzzese E, Mologni L, Sinibaldi-Salimei P, de Fabritis P, Gambacorti-Passerini C, Amadori S, Birge RB. (2011)

Exp Hematol. 39(6):653-665.e6. IF 3,08. Citazioni: 37

P De Wispelaere, A Van Soom, T Rijsselaere, J Saunders, G Storms, A Gambacurta, F. Haesebrouck .(2011)

Tijdschrift Voor Diergeneeskunde 136 (6), 418-420. IF 0,188. Citazioni: -

Di Pietro V, Gambacurta A, Amorini AM, Finocchiaro A, D'Urso S, Ceccarelli L, Tavazzi B, Giardina B, Lazzarino G(2008).

Clin Biochem. 41(7-8):611-5. IF 3,28. Citazioni: 5

C Ciaccio, A Gambacurta, G. de Sanctis, D Spagnolo, C Sakarikou, M. Coletta. (2006).

Biochem J. 15;395(2):295-301. IF 4,4. Citazioni: 7

A Gambacurta, L Fiorucci, P Basili, F Erba, A Amoresano, F Ascoli. (2003).

Eur J Biochem. 270(3):507-17. Title changed to 'FEBS Journal' since2005IF 4,67. Citazioni: 3

S Della Longa, A Gambacurta, A Bertollini, M Girasole, A Castellano. (2001)

European Biophys J. 2001;29(8):559-68. IF 1,733. Citazioni: 2

A Gambacurta, P Basili, F Ascoli. (2000)

Gene. 2000 Sep 5;255(1):75-81. IF 3,47. Citazioni: 3

JBIC Journal of Biological Inorganic Chemistry 4 (6), 678-683IF 3,2. Citazioni: 2

S Della Longa, A Gambacurta, I Ascone, A Bertollini, M Girasole. (1999)

Journal Of Synchrotron Radiation 6 (3), 392-393IF 0,67. Citazioni: 2

A Pardanani, A Gambacurta, F Ascoli, W Royer Jr. (1998)

Journal of Molecular Biology 284 (3), 729-739IF 5,1. Citazioni: 29

M Falconi, A Desideri, A Cupane, M Leone, G Ciccotti, Es Peterson, A.Gambacurta, F. Ascoli. (1998)

Biophysical Journal 75 (5), 2489-2503IF 3,4. Citazioni: 6

Mc Piro, A Gambacurta, P Basili, F Ascoli. (1998)

Gene 221 (1), 45-49IF 3,47. Citazioni: 2

A Gambacurta, Mc Piro, F Ascoli. (1998)

Journal Of Molecular Evolution 47 (2), 167-171IF 2,3. Citazioni: 4

Y Wu, M Basti, A Gambacurta, E Chiancone, F Ascoli, G. La Mar. (1996)

Biochimica et Biophysica Acta (Bba)-Protein Structure And Molecular Enzymology 8, 94IF 3,0. Citazioni: 11

Mc Piro, A Gambacurta, F Ascoli. (1996)

Journal Of Molecular Evolution 43 (6), 594-601IF 2,3. Citazioni: 10

M Pallaoro, A Gambacurta, L Fiorucci, G Mignogna, D Barra, F Ascoli. (1996)

European Journal Of Biochemistry 237 (1), 100-105IF 4,67. Citazioni: 19

Gambacurta A., Piro M.C., Coletta M., Clementi M.E., Polizio F., Desideri A., Santucci R., Ascoli F. (1995).

J. Mol. Biol, 248, 910-917. IF 5,1. Citazioni: 10

Gambacurta, A., Piro, M.C., Ascoli, F.(1993)

BBA - Gene Structure and Expression, 1174 (3), pp. 267-273. IF 4,72. Citazioni: 2

Gambacurta, A., Piro, M.C., Ascoli, F. (1993)

FEBS Letters, 330 (1), pp. 90-94. IF 4,12. Citazioni: 23

A Floridi, A Gambacurta, A Bagnato, C Bianchi, Mg Paggi, B Silvestrini. (1988)

Experimental and Molecular Pathology 49 (3), 421-431IF 3,3. Citazioni: 23

A Floridi, C Bianchi, A Bagnato, A Gambacurta, Mg Paggi, B Silvestrini. (1987)

Anticancer Research 7 (6), 1149-1152IF 2,4. Citazioni:

“Partial Loss of Function ABCA12 Mutations Generate Reduced Deposition of Glucosyl-Ceramide, Leading to Patchy Ichthyosis and Erythrodermia Resembling Erythrokeratodermia Variabilis et Progressiva (EKVP)”
1. “In Vivo Identification of H3K9me2/H3K79me3 as an Epigenetic Barrier to Carcinogenesis”.
1. “DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines”.
1. “Opening the Gate to the Serism Project: From Earth to Space and Back”.
1. “Microcalcifications Drive Breast Cancer Occurrence and Development by Macrophage-Mediated Epithelial to Mesenchymal Transition”.
1. “Cell death in cancer in the era of precision medicine”
1. “Human osteogenic differentiation in Space: proteomic and epigenetic clues to better understand osteoporosis”
1. “Human endogenous retrovirus K in the crosstalk between cancer cells microenvironment and plasticity: a new perspective for combination therapy”
1. “Challenging tumor resistance with less toxic, more effective drug combinations: an example from neuroblastoma”.
1. “Myoblasts rely on TAp63 to control basal mitochondria respiration”
1. “TAp73 regulates ATP7A: possible implications for ageing-related diseases”.
1. “Rapid Rapamycin-Only Induced Osteogenic Differentiation of Blood-Derived Stem Cells and Their Adhesion to Natural and Artificial Scaffolds”.
1. “HERV-K activation is strictly required to sustain CD133+ melanoma cells with stemness features”.
1. “Back on Track: New Perspectives on Cancer Cell Reprogramming”.
1. “Differentiation of human neuroblastoma cells towards the osteogenic lineage by mTOR inhibitor”.
1. “Blood-Derived Stem Cells (BDSCs) Plasticity: In Vitro Hepatic Differentiation”.
1. “IL-25 prevents and cures fulminant hepatitis in mice through a myeloid-derived suppressor cell-dependent mechanism”.
1. “A new clinical approach: use of blood-derived stem cells (BDSCs) for superficial digital flexor tendon injuries in horses”.
1. “Blood derived stem cells: An ameliorative therapy in veterinary ophthalmology”.
1. “A Multicancer-Like Syndrome in A Dog Characterized By P53 and Cell Cycle-Checkpoint Kinase 2 (Chk2) Mutations and Sirtuin Gene (Sirt1) Down-Regulation”.
1. “The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise”.
1. “ATP Regulation of the Ligand-Binding Properties in Temperate and Cold-Adapted Haemoglobins. X-Ray Structure And Ligand-Binding Kinetics In The Sub-Antarctic Fish Eleginops”
1. “Purification and expansion of stem cells from equine peripheral blood, with clinical applications”
1. “TCTP is a critical survival factor that protects cancer cells from oxidative stress-induced cell-death”.
1. “Sphingosine Kinase 1 Overexpression Is Regulated By Signaling Through Pi3k, Akt2, And mTOR In Imatinib-Resistant Chronic Myeloid Leukemia Cells”.
1. “Ultrasound Prediction Of The Partus Datum In Dogs”
1. “A new T677C mutation of the aspartoacylase gene encodes for a protein with no enzymatic activity”
1. “rhEPO (Recombinant Human Eosinophil Peroxidase): Expression in Pichia Pastoris and Biochemical Characterization”
1. “Bovine Tryptases: cDNA cloning, tissue specific expression and characterization of the lung isoform”
1. “Structure of the Fe-Heme In The Homodimeric Hemoglobin From Scapharca Inaequivalvis And In The T72i Mutant: An X-Ray absorption spectroscopic study at low temperature”.
1. “Scapharca Inaequivalvis A And B Miniglobin Genes: Promoter Activity Of The 5′ Flanking Regions And In Vivo Transcription”.
1. “Functional Modulation of The Thr72→Ile Mutant From Scapharca Inaequivalvis homodimeric Hemoglobin”. M Coletta, A Gambacurta, Me Clementi, F Erba, F Polizio, M Falconi , F. Ascoli (1999)
1. “Fe-Heme Structure And Dynamics In Thr72→ Ile Mutant Scapharca Inaequivalvis Hemoglobin By X-Ray Absorption Spectroscopy”.
1. “Mutational destabilization of the critical interface water cluster in scapharca dimeric hemoglobin: structural basis for altered allosteric activity”.
1. “Structural And Dynamic Properties Of The Homodimeric Hemoglobin From Scapharca Inaequivalvis Thr-72→ Ile Mutant: Molecular Dynamics Simulation, Low Temperature Visible”.
1. “The Exon/Intron Organization Of The Globin Gene Of Scapharca Inaequivalvis Homodimeric Hemoglobin: Unusual Intron Homology With Other Bivalve Mollusc Globin Genes”.
1. “Scapharca Inaequivalvis Tetrameric Hemoglobin A and B Genes: Evidence for a Minigene”.
1. “Proton-NMR Investigation Of The Heme Cavity In The Cyanomet Derivative Of The Cooperative Homodimeric Hemoglobin From Scapharca Inaequivalvis”.
1. “Scapharca Inaequivalvis Tetrameric Hemoglobin A and B Chains: cDNA Sequencing and Genomic Organization”.
1. “cDNA cloning and primary structure of tryptase from bovine mast cells, and evidence for the expression of bovine pancreatic trypsin inhibitor mRNA in the same cells”.
1. “A single mutation (Thr72 → IIe) at the subunit interface is crucial for the functional properties of the homodimeric co-operative haemoglobin from Scapharca inaequivalvis”.
1. “Differential in vitro translation of the precursors of bovine pancreatic trypsin inhibitor and its isoinhibitor II is controlled by the 5′-end region of their mRNAs”.
1. “Cooperative homodimeric hemoglobin from Scapharca inaequivalvis. cDNA cloning and expression of the fully functional protein in E. Coli “.
1. “Modulation of Adriamycin Uptake By Lonidamine In Ehrlich Ascites Tumor Cells”.
1. “Lonidamine-Induced Outer Membrane Permeability and Susceptibility of Mitochondria to inhibition by Adriamycin”.

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