Francesca Bernassola

Qualifica
ASSOCIATO CONFERMATO
Fonte dei dati: Archivio della Ricerca http://art.torvergata.it
  1. Gatti, V., Bernassola, F., Talora, C., Melino, G., & Peschiaroli, A. (2020). The Impact of the Ubiquitin System in the Pathogenesis of Squamous Cell Carcinomas. CANCERS, 12(6), 1595 [10.3390/cancers12061595]. Dettagli
  2. D'Amico, S., Krasnowska, E., Manni, I., Toietta, G., Baldari, S., Piaggio, G., et al. (2020). DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines. CELLS, 9(5), 1260. Dettagli
  3. Melino, G., CECCONI, F., Giuseppe Pelicci, P., Wah Mak, T., & BERNASSOLA, F. (2019). Emerging roles of HECT-type E3 ubiquitin ligases in autophagy regulation. MOLECULAR ONCOLOGY, 13, 2033-2048 [10.1002/1878-0261.12567]. Dettagli
  4. Frezza, V., Fierro, C., Gatti, E., Peschiaroli, A., Lena, A., Petruzzelli, M., et al. (2018). ΔNp63 promotes IGF1 signalling through IRS1 in squamous cell carcinoma. AGING, 10(12), 4224-4240 [10.18632/aging.101725]. Dettagli
  5. Sanarico, A.g., Ronchini, C., Croce, A., Memmi, E.m., Cammarata, U.a., De Antoni, A., et al. (2018). The E3 ubiquitin ligase WWP1 sustains the growth of acute myeloid leukaemia. LEUKEMIA, 32(4), 911-919-919 [10.1038/leu.2017.342]. Dettagli
  6. Agostini, M., Melino, G., & Bernassola, F. (2018). The p53 Family in Brain Disease. ANTIOXIDANTS & REDOX SIGNALING, 29(1), 1-14. Dettagli
  7. Galluzzi, L., Vitale, I., Aaronson, S.a., Abrams, J.m., Adam, D., Agostinis, P., et al. (2018). Molecular mechanisms of cell death: Recommendations of the Nomenclature Committee on Cell Death 2018. CELL DEATH AND DIFFERENTIATION, 25(3), 486-541 [10.1038/s41418-017-0012-4]. Dettagli
  8. Chillemi, G., Kehrloesser, S., Bernassola, F., Desideri, A., Dotsch, V., Levine, A., et al. (2017). Structural evolution and dynamics of the p53 proteins. COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 7(4), a028308. Dettagli
  9. Lenti, E., Farinello, D., Yokoyama, K., Penkov, D., Castagnaro, L., Lavorgna, G., et al. (2016). Transcription factor TLX1 controls retinoic acid signaling to ensure spleen development. THE JOURNAL OF CLINICAL INVESTIGATION, 126, 2452-2464 [10.1172/JCI82956]. Dettagli
  10. Melino, G., Memmi, E.M., Pelicci, P.G., & Bernassola, F. (2015). Maintaining epithelial stemness with p63. SCIENCE SIGNALING, 8(387), re9. Dettagli
  11. Memmi, E., Sanarico, A., Giacobbe, A., Peschiaroli, A., Frezza, V., Cicalese, A., et al. (2015). p63 sustains self-renewal of mammary cancer stem cells through regulation of Sonic Hedgehog signaling. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(11), 3499-3504. Dettagli
  12. Amelio, I., Antonov, A., Catani, M.V., Massoud, R., Bernassola, F., Knight, R., et al. (2014). TAp73 promotes anabolism. ONCOTARGET, 5(24), 12820-934. Dettagli
  13. Landré V, R.B. (2014). Screening for E3-Ubiquitin ligase inhibitors: challenges and opportunities. ONCOTARGET, 5(18), 7988-8013 [10.18632/oncotarget.2431]. Dettagli
  14. Candi, E., Agostini, M., Melino, G., & Bernassola, F. (2014). How the TP53 Family Proteins TP63 and TP73 contribute to tumorigenesis: Regulators and effectors. HUMAN MUTATION, 35(6), 702-714 [10.1002/humu.22523]. Dettagli
  15. Rossi, M., Rotblat, B., Ansell, K., Amelio, I., Caraglia, M., Misso, G., et al. (2014). High throughput screening for inhibitors of the HECT ubiquitin E3 ligase ITCH identifies antidepressant drugs as regulators of autophagy. CELL DEATH & DISEASE, 5(5), e1203 [10.1038/cddis.2014.113]. Dettagli

    10.1038/cddis.2014.113

    Citazione
    Rossi, M., Rotblat, B., Ansell, K., Amelio, I., Caraglia, M., Misso, G., et al. (2014). High throughput screening for inhibitors of the HECT ubiquitin E3 ligase ITCH identifies antidepressant drugs as regulators of autophagy. CELL DEATH & DISEASE, 5(5), e1203 [10.1038/cddis.2014.113].
    Data
    2014
    id
    PUBBLICAZIONE_231854
    Tipologia
    Articolo su rivista
    Categoria Isi-Crui
    Antidepressive Agents; Autophagy; Binding Sites; Cell Line, Tumor; Clomipramine; Drug Synergism; Enzyme Inhibitors; High-Throughput Screening Assays; Humans; Models, Molecular; Protein Structure, Tertiary; Reproducibility of Results; Ubiquitin; Ubiquitin-Protein Ligases
    SSD
    Settore BIO/10
    metadata4
    Inhibition of distinct ubiquitin E3 ligases might represent a powerful therapeutic tool. ITCH is a HECT domain-containing E3 ligase that promotes the ubiquitylation and degradation of several proteins, including p73, p63, c-Jun, JunB, Notch and c-FLIP, thus affecting cell fate. Accordingly, ITCH depletion potentiates the effect of chemotherapeutic drugs, revealing ITCH as a potential pharmacological target in cancer therapy. Using high throughput screening of ITCH auto-ubiquitylation, we identified several putative ITCH inhibitors, one of which is clomipramine--a clinically useful antidepressant drug. Previously, we have shown that clomipramine inhibits autophagy by blocking autophagolysosomal fluxes and thus could potentiate chemotherapy in vitro. Here, we found that clomipramine specifically blocks ITCH auto-ubiquitylation, as well as p73 ubiquitylation. By screening structural homologs of clomipramine, we identified several ITCH inhibitors and putative molecular moieties that are essential for ITCH inhibition. Treating a panel of breast, prostate and bladder cancer cell lines with clomipramine, or its homologs, we found that they reduce cancer cell growth, and synergize with gemcitabine or mitomycin in killing cancer cells by blocking autophagy. We also discuss a potential mechanism of inhibition. Together, our study (i) demonstrates the feasibility of using high throughput screening to identify E3 ligase inhibitors and (ii) provides insight into how clomipramine and its structural homologs might interfere with ITCH and other HECT E3 ligase catalytic activity in (iii) potentiating chemotherapy by regulating autophagic fluxes. These results may have direct clinical applications.
    Tutti gli Autori
    High throughput screening for inhibitors of the HECT ubiquitin E3 ligase ITCH identifies antidepressant drugs as regulators of autophagy
    tipologia
    Rossi, M; Rotblat, B; Ansell, K; Amelio, I; Caraglia, M; Misso, G; Bernassola, F; Cavasotto, Cn; Knight, Ra; Ciechanover, A; Melino, G
  16. Giacobbe, A., Bongiorno-Borbone, L., Bernassola, F., Terrinoni, A., Markert, E., Levine, A., et al. (2013). p63 regulates glutaminase 2 expression. CELL CYCLE, 12(9), 1395-1405 [10.4161/cc.24478]. Dettagli
  17. Celardo, I., Grespi, F., Antonov, A., Bernassola, F., Garabadgiu, A., Melino, G., et al. (2013). Caspase-1 is a novel target of p63 in tumor suppression. CELL DEATH & DISEASE, 4, e645-e645 [10.1038/cddis.2013.175]. Dettagli
  18. Peschiaroli, A., Scialpi, F., Bernassola, F., El Sherbini el, S., & Melino, G. (2010). he E3 ubiquitin ligase WWP1 regulates ΔNp63-dependent transcription through Lys63 linkages. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 402(2), 425-430. Dettagli
  19. Peschiaroli, A., Scialpi, F., Bernassola, F., El Sherbini, E.S., & Melino, G. (2010). The E3 ubiquitin ligase WWP1 regulates ΔNp63-dependent transcription through Lys63 linkages. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 402(2), 425-430 [10.1016/j.bbrc.2010.10.050]. Dettagli
  20. Busuttil, V., Droin, N., McCormick, L., Bernassola, F., Candi, E., Melino, G., et al. (2010). NF-kappaB inhibits T-cell activation-induced, p73-dependent cell death by induction of MDM2. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(42), 18061-18066 [10.1073/pnas.1006163107]. Dettagli
  21. Busuttil, V., Droin, N., McCormick, L., Bernassola, F., Candi, E., Melino, G., et al. (2010). NF-kappaB inhibits T-cell activation-induced, p73-dependent cell death by induction of MDM2. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107(42), 18061-18066 [10.1073/pnas.1006163107]. Dettagli
  22. Dötsch, V., Bernassola, F., Coutandin, D., Candi, E., & Melino, G. (2010). p63 and p73, the ancestors of p53, 2(9), a004887 [10.1101/cshperspect.a004887]. Dettagli
  23. Dötsch, V., Bernassola, F., Coutandin, D., Candi, E., & Melino, G. (2010). p63 and p73, the ancestors of p53. COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2(9), a004887 [10.1101/cshperspect.a004887]. Dettagli
  24. Bernassola, F., Ciechanover, A., & Melino, G. (2010). The ubiquitin proteasome system and its involvement in cell death pathways. CELL DEATH AND DIFFERENTIATION, 17(1), 1-3 [10.1038/cdd.2009.189]. Dettagli
  25. Peschiaroli, A., Scialpi, F., Bernassola, F., Pagano, M., & Melino, G. (2009). The F-box protein FBXO45 promotes the proteasome-dependent degradation of p73. ONCOGENE, 28(35), 3157-3166. Dettagli
  26. Bernassola, F., Karin, M., Ciechanover, A., & Melino, G. (2008). The HECT Family of E3 Ubiquitin Ligases: Multiple Players in Cancer Development. CANCER CELL, 14(1), 10-21. Dettagli
  27. Melino G., G.E. (2008). Itch: A HECT-type E3 ligase regulating immunity, skin and cancer. CELL DEATH AND DIFFERENTIATION, 15(7), 1103-1112 [10.1038/cdd.2008.60]. Dettagli
  28. Raimondo, D., Giorgetti, A., Bernassola, F., Melino, G., & Tramontano, A. (2008). Modelling and molecular dynamics of the interaction between the E3 ubiquitin ligase itch and the E2 UbcH7. BIOCHEMICAL PHARMACOLOGY, 76(11), 1620-1627. Dettagli
  29. Bellodi, C., Kindle, K., Bernassola, F., Cossarizza, A., Dinsdale, D., Melino, G., et al. (2006). A cytoplasmic PML mutant inhibits p53 function. CELL CYCLE, 5(22), 2688-2692 [10.4161/cc.5.22.3504]. Dettagli
  30. Bellodi, C., Kindle, K., Bernassola, F., Dinsdale, D., Cossarizza, A., Melino, G., et al. (2006). Cytoplasmic function of mutant promyelocytic leukemia (PML) and PML-retinoic acid receptor-α. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 281(20), 14465-14473. Dettagli
  31. Bernassola, F., Oberst, A., Melino, G., & Pandolfi, P. (2005). The promyelocytic leukaemia protein tumour suppressor functions as a transcriptional regulator of p63. ONCOGENE, 24(46), 6982-6986. Dettagli
  32. Bernassola, F., Salomoni, P., Oberst, A., Di Como, C.J., Pagano, M., Melino, G., et al. (2004). Ubiquitin-dependent degradation of p73 is inhibited by PML. JOURNAL OF EXPERIMENTAL MEDICINE, 199(11), 1545-1557 [10.1084/jem.20031943]. Dettagli
  33. Lovat, P.E., Oliverio, S., Ranalli, M., Corazzari, M., Rodolfo, C., Bernassola, F., et al. (2002). GADD153 and 12-lipoxygenase mediate fenretinide-induced apoptosis of neuroblastoma. CANCER RESEARCH, 62(18), 5158-5167. Dettagli
  34. Bernassola, F., Boumis, G., Corazzari, M., Bertini, G., Citro, G., Knight, R.A., et al. (2002). Osmotic resistance of high-density erythrocytes in transglutaminase 2-deficient mice. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 291(5), 1123-1127. Dettagli
  35. Bernassola, F., Federici, M., Corazzari, M., Terrinoni, A., Hribal, M.l., De Laurenzi, V., et al. (2002). Role of transglutaminase 2 in glucose tolerance: Knockout mice studies and a putative mutation in a MODY patient, 16(11), 1371-1378 [10.1096/fj.01-0689com]. Dettagli
  36. Mastroberardino, P.G., Iannicola, C., Nardacci, R., Bernassola, F., De Laurenzi, V., Melino, G., et al. (2002). 'Tissue' transglutaminase ablation reduces neuronal death and prolongs survival in a mouse model of Huntington's disease. CELL DEATH AND DIFFERENTIATION, 9(9), 873-880 [10.1038/sj.cdd.4401093]. Dettagli
  37. Bernassola, F., Catani, M.V., Corazzari, M., Rossi, A., & Melino, G. (2001). Inactivation of multiple targets by nitric oxide in CD95-triggered apoptosis. JOURNAL OF CELLULAR BIOCHEMISTRY, 82(1), 123-133. Dettagli
  38. Annicchiarico-Petruzzelli, M., Bernassola, F., Lovat, P.E., Redfern, C., Pearson, A., & Melino, G. (2001). Apoptosis in neuroblastomas induced by interferon-gamma involves the CD95/CD95L pathway. In MEDICAL AND PEDIATRIC ONCOLOGY (pp.115-117). NEW YORK : WILEY-LISS. Dettagli
  39. Bernassola, F., Catani, M.V., Corazzari, M., Rossi, A., & Melino, G. (2001). Inactivation of multiple targets by nitric oxide in CD95-triggered apoptosis. JOURNAL OF CELLULAR BIOCHEMISTRY, 123-133. Dettagli
  40. Vieira, H., Belzacq, A.S., Haouzi, D., Bernassola, F., Cohen, I., Jacotot, E., et al. (2001). The adenine nucleotide translocator: a target of nitric oxide, peroxynitrite, and 4-hydroxynonenal. ONCOGENE, 20(32), 4305-4316. Dettagli
  41. Lovat, P.E., Ranalli, M., Bernassola, F., Tilby, M., Malcolm, A.J., Pearson, A., et al. (2000). Synergistic induction of apoptosis of neuroblastoma by fenretinide or CD437 in combination with chemotherapeutic drugs. INTERNATIONAL JOURNAL OF CANCER, 88(6), 977-985. Dettagli
  42. Lovat, P.E., Ranalli, M., Annichiarrico-Petruzzelli, M., Bernassola, F., Piacentini, M., Malcolm, A.J., et al. (2000). Effector mechanisms of fenretinide-induced apoptosis in neuroblastoma. EXPERIMENTAL CELL RESEARCH, 260(1), 50-60. Dettagli
  43. Lovat, P.E., Ranalli, M., Bernassola, F., Tilby, M., Malcolm, A.J., Pearson, A., et al. (2000). Distinct properties of fenretinide and CD437 lead to synergistic responses with chemotherapeutic reagents. In Medical and Pediatric Oncology (pp.663-668). NEW YORK : WILEY-LISS. Dettagli
  44. Melino, G., Bernassola, F., Catani, M.V., Rossi, A., Corazzari, M., Sabatini, S., et al. (2000). Nitric oxide inhibits apoptosis via AP-1-dependent CD95L transactivation. CANCER RESEARCH. Dettagli
  45. Melino, G., Catani, M.V., Corazzari, M., & Bernassola, F. (2000). Nitric oxide can inhibit apoptosis or switch it into necrosis. CELLULAR AND MOLECULAR LIFE SCIENCES. Dettagli
  46. Rossi, A., Catani, M.V., Candi, E., Bernassola, F., Puddu, P., & Melino, G. (2000). Nitric oxide inhibits cornified envelope formation in human keratinocytes by inactivating transglutaminases and activating protein 1. JOURNAL OF INVESTIGATIVE DERMATOLOGY. Dettagli
  47. Bernassola, F., Rossi, A., & Melino, G. (1999). Regulation of transglutaminases by nitric oxide. In MECHANISMS OF CELL DEATH - THE SECOND ANNUAL CONFERENCE OF THE CELL DEATH SOCIETY (pp.83-91). NEW YORK : NEW YORK ACAD SCIENCES. Dettagli
  48. Bernassola, F., Scheuerpflug, C., Herr, I., Krammer, P.H., Debatin, K.M., & Melino, G. (1999). Induction of apoptosis by IFN gamma in human neuroblastoma cell lines through the CD95/CD95L autocrine circuit. CELL DEATH AND DIFFERENTIATION, 6(7), 652-660. Dettagli
  49. Catani, M.V., Bernassola, F., Rossi, A., & Melino, G. (1998). Inhibition of clotting factor XIII activity by nitric oxide. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 249(1), 275-278. Dettagli